Journal: Journal of Lipid Research

Article Title: Endothelial SR-B1 is dispensable for thermogenesis but promotes selective cholesterol uptake in brown adipose tissue

doi: 10.1016/j.jlr.2025.100894

Figure Lengend Snippet: Impact of endothelial SR-B1 on organ-specific glucose and lipid handling in response to cold exposure and HFD feeding. A–D: A postprandial tolerance test was performed by orally applying a glucose-lipid emulsion that was traced with 3 H-triolein and 14 C-DOG. Two hours after gavage, mice were sacrificed, and radioactivity was determined in different organs. Data are presented as mean values per organ. Different letters indicate statistical difference P < 0.05. A and B: Male Scarb1 fl/fl -Cdh5 Cre+ and Scarb1 fl/fl -Cdh5 Cre − mice on a chow diet were housed at room temperature or 6°C for 24 h before the postprandial challenge (n = 5). Uptake of (A) TRL-derived 3 H-labeled triolein/oleic acid and (B) 14 C-DOG by different organs. C, D, F: Male Scarb1 fl/fl -Cdh5 Cre+ and Scarb1 fl/fl -Cdh5 Cre − mice were fed an HFD for 4 weeks and afterward housed at room temperature or 6°C for 24 h before the oral glucose fat tolerance test (n = 6). Uptake of (C) TRL-derived 3 H-labeled triolein/oleic acid and (D) 14 C-DOG. E: Female Scarb1 fl/fl -Cdh5 Cre+ and Scarb1 fl/fl -Cdh5 Cre − mice were housed at room temperature or 6°C (cold) for 24 h (n = 4) and fed a chow diet. E and F: Expression data of lipoprotein receptors and glucose transporters were determined in BAT isolated from chow (E) or (F) HFD-fed mice and normalized to Tbp . Data are presented as mean values ± SEM, and room temperature Scarb1 fl/fl -Cdh5 Cre − group was set to 1. Different letters indicate statistical difference P < 0.05. Cd36 (cluster of differentiation 36); Glut1 (glucose transporter 1), Glut4 (glucose transporter 4); Ldlr (low-density lipoprotein receptor); Lrp1 (LDL receptor-related protein-1); Scarb1 (scavenger receptor class B1); Scarb2 (scavenger receptor class B2, also known as lysosomal integral membrane protein 2); Vldlr (very-low-density lipoprotein receptor). Please note that all data points are plotted, but some symbols coincide, which gives the impression of a fewer n number.

Article Snippet: Gene expression was measured by QuantstudioTM 5 Real-Time PCR Systems (ThermoFisher Scientific), and the following TaqMan® Assay-on-demand primer sets were used: Tbp : Mm00446973_m1, Scarb1 : Mm01198172_m1, Ucp1 : Mm00494069_m1, Elovl3: Mm00468164_m1, Ppargc1a : Mm00447183_m1, Fasn : Mm00662319_m1, Dio2 : Mm00515664_m1, Lpl : Mm00434764_m1, Cd36 : Mm00432403_m1, Scarb2 : Mm00446977_m1, Glut1 : Mm00441480_m1, Glut4 : Mm01245502_m1, Ldlr : Mm00440169_m1; Lrp1 : Mm00464608_m1, Vldlr : Mm00443281_m1, Emr1 : Mm00802530_m1, Gpihbp1 : Mm01205849_g1, 36B4: Hs99999902_m1, ADIPOQ: Hs00605917_m1, GPIHBP1: Hs01564843_m1, EMR1: Hs00173562_m1, and SCARB1: Hs00969821_m1, VLDLR: Hs00182461_m1.

Techniques: Emulsion, Radioactivity, Derivative Assay, Labeling, Expressing, Isolation, Membrane

Journal: Asian Journal of Pharmaceutical Sciences

Article Title: Targeted delivery of glucocerebrosidase to lysosomes: The LYSOTAC (LYSOsome-TArgeting Chimera) technology

doi: 10.1016/j.ajps.2026.101149

Figure Lengend Snippet: LYSOTAC partially recovers GCase activity in LIMP-2 deficient HEK293 cells. (A) Scheme of generating SCARB2 -/- clones (Created in BioRender. Park, J. (2026) https://BioRender.com/6qf7ykt ); (B) WB of HEK293 cell clones transfected with gRNA vector targeting SCARB2 ; Relative quantification of (C) LIMP-2 and (D) GCase compared to control HEK293 cells in (B) ( n = 5); (E) GCase activity of HEK293 cell clones transfected with gRNA vector targeting SCARB2 ( n = 5); GCase activity of LIMP-2 deficient HEK293 cells (clone #2) exposed to (F) ATB2057 or (G) ATB2058 for 7 d was compared with control HEK293 cells ( n = 5); (H, I) Increased GCase activity of LIMP-2 deficient HEK293 cells in (F) and (G) is shown as a percentage change ( n = 5); (J) GCase activity of LIMP-2 deficient HEK293 cells treated with ATB2057 (1 µM), ATB1021 (1 µM), JZ-4109 (1 µM), and ambroxol (1 or 50 µM) for 7 d. Differences between groups were evaluated by one-way ANOVA (C, D, E, H, I, and J) or two-way ANOVA (F and G). The data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: LIMP-2-deficient HEK293 cells were produced using a LIMPII (SCARB2) Human Gene Knockout Kit (CRISPR) (# KN402162 ; Origene, Rockville, MD, USA) according to the manufacturer’s instructions.

Techniques: Activity Assay, Clone Assay, Transfection, Plasmid Preparation, Quantitative Proteomics, Control